Disease Indexing for Banana and Citrus


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Disease Indexing for Banana and Citrus:
A Research and Service Facility for Cagayan Valley
First International Conference on Food, Environment, and Culture Hotel Supreme, Magsaysay Avenue, Baguio City May 15-18, 2017

General Information

Project Title:

Disease Indexing for Banana and Citrus: A Research and Service Facility for Cagayan Valley

R&D Workers: EASana, MCSalas, GADizon, ALEsguerra, JMGuillermo, DPBagnes, and FMNabulneg

Funding:

DOST-PCAARRD and NVSU

Duration: Budget:

Two years (Feb 2015-Feb 2017)

DOST-PCAARRD: 4.69 M

NVSU:

300 k

Disease Indexing for Banana and Citrus: A Research and Service Facility for Cagayan Valley

Problem

Solution

Output

 20-75% BBTV incidence
 40-60% HLB and CTV incidence

Disease Indexing
5M
1st in the region and 4th in the country

Quality planting materials and service for TCL, Nurseries, and Farms
Impact

OBJECTIVES
 Enhance capability of NVSU for serology and PCR detection of BBTV, BMV, HLB, and CTV;
 Provide disease indexing service to banana and citrus growers as a component of a quality planting materials and disease management system
 Equip at least two farmer groups in improving banana and citrus production and nursery management;

OBJECTIVES
 Establish baseline information on the current production practices and management of pests and diseases of banana in Nueva Vizcaya.
 Map well managed citrus orchards and budwood source mother trees in Nueva Vizcaya

METHODOLOGY
Main Activities:
(1) Facilities Enhancement
 Repairs and fixtures in the laboratory
 Procurement of new equipment for disease indexing

METHODOLOGY
(1) Facilities Enhancement

2 old donated Thermal Cyclers

1 Gel Doc System

1 Gel Electrophoresis System

METHODOLOGY
(2) Disease Indexing Service

Present Disease Indexing Application:
Plate Trap Enzyme Linked Antigen Immunosorbent Assay (PTA-ELISA) for BBTV and CTV

Enhanced Application:
 PTA-ELISA for BMV and CTV
 DNA-Based detection for BBTV and HLB

METHODOLOGY

(2) Disease Indexing Service
PCR-Based Disease Detection (Su et al 2007)

For BBTV:
C1-CR F 5’-GGA AGA AGC CTC TCA TCT GCT TCA GAG AGC-3’ R 5’-CAG GCG CAC ACC TTG AGA AAC GAA AGG GAA-3’

S-CR

F 5’-GGG GCT TAT TAT TAC CCC CAG C-3’ R 5’-AGC GCT TAC GTG GCG CAG CAC TAA CT-3’

SR-CR F 5’-TGT CGT CGG CGA CGA AGT CG-3’ R 5’- GGA CAT CCT CCT CCT TCA GAA GAG AGA-3’

For HLB:

P1

F 5’-CAC CGA AGA TAT GGA CAA CA-3’

R 5’-GAG GTT CTT GTG GTT TTT CTG-3’

METHODOLOGY
(2) Disease Indexing Service

NVSU Disease Indexing Facility
NVSU Banana Tissue Culture and Nursery
NVSU Citrus Foundation Block

Tissue Culture Labs and Nurseries in RII
Banana and Citrus Farms
Banana and Citrus Nurseries

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Disease Indexing for Banana and Citrus